Other

Part:BBa_K3454000:Design

Designed by: Mingxuan Chi   Group: iGEM20_ShanghaiTech_China   (2020-10-21)


MCR-1_crRNA_A_Synthesis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is designed for Cas12a. The T7 promoter and scaffold RNA is common among different crRNA synthesis, which means you do not need to change them while using.



Source

It does not come from genomic DNA. It is artificially synthesized.


References